Wholesale Distributor Inquiries Welcomed!

Aseptic Manufacturing and Bottling Process: A Brief Description

While the manufacturing methods for H2O-BOO2STŪ EQUINE OXYGEN is a very tightly held proprietary process, there are specific things that can be revealed to indicate the general concepts and to establish that thorough GMP practices are in place to assure quality and to minimize potential contamination.

H2O-BOO2STŪ EQUINE OXYGEN is created from a basic distilled water and sodium chloride base solution. A 220 volt regulated current is passed between a cathode and anode submersed in the solution. The exact modality of the solution, the exact current that is applied, the length of time the solution is processed, the exact dimensions and composition of the electrodes, as well as their depth in the saline solution, are proprietary information and considered trade secrets.

As the current passes between the electrodes, the electron stream splits the water molecules in the solution creating H2 and O- molecules. The hydrogen gas (H2) is released at the anode through a one-way valve. The O- is further processed creating a stable molecule of polyatomic tetraoxygen (O4). The temperature in the processing boiler reaches temperatures in excess of 140š C for a period of not less than eighty (80) minutes. Again, the exact temperature and the length of time the solution is processed is a trade secret.

When a specific time has transpired, the processed solution is passed through a two-step activated charcoal filtering system to remove any residues, particulates and possible contaminates. Some sodium and chloride is removed as well from the processed solution. The solution is gravity drained into fifty-five (55) gallon FDA approved pharmaceutical grade sterilized HDPE poly drums and these drums are immediately sealed and transported from the laboratory to the bottling facility.

At the bottling facility, aseptic and sterilized hoses are attached to the drum and to the digital bottle filling equipment. Between the drum and the filling heads is a two-stage UV germicidal system. The first stage uses low pressure mercury vapor lamps which produce ultraviolet rays that are lethal to micro organisms. Radiations are generated by electrical discharge through low pressure inert gases and mercury vapor within the special quartz tube of the lamp. Approximately 95% of the UV rays from the lamp are at the wave length of 2537A (commonly referred to as 254 nm) which is the region of maximum bactericidal effectiveness. A second stage uses germicidal lamps designed to produce controlled amounts of a shorter wave length of ultraviolet measured at 1850 Angstrom units (commonly referred to as 185 nm). This wavelength generates ozone, which is a form of oxygen, and is recognized as an extremely effective germicidal agent.

The H2O-BOO2STŪ EQUINE OXYGEN leaves the second stage and is transferred by gravity to the stainless steel filling heads. The bottles are filled with H2O-BOO2STŪ EQUINE OXYGEN to specification, immediately capped, labeled and sealed for protection. From there bottles go to the packaging area where they are immediately placed in 200 pound kraft boxes, separated by chip cell dividers, and the boxes are sealed.

All processes are FDA, EPA and OSHA approved for dietary supplements and food processing and handling.


A Brief Explanation of the Manufacturing Process 

H2O-BOO2STŪ EQUINE OXYGEN is manufactured in an F.D.A. approved GMP laboratory designated for the manufacturing of dietary supplements. The process involves the disassociation of oxygen molecules from water molecules in an electrolytic bath containing a mixture of water and sodium chloride. A regulated current is then applied to two proprietary composite metal electrodes. The hydrogen gas is released and the oxygen molecules are stabilized in the electrolytic solution. What makes H2O-BOO2STŪ EQUINE OXYGEN unique is that neither oxychlorine (chlorine dioxide) nor peroxide molecules are formed keeping the pH of the final solution in the 7.0 to 8.5 range. Yet, sufficient oxygen remains in solution for an extended period of time to retain its bioavailable and biocidal properties. Mineral analysis and other studies are available upon written request. A statement of non-disclosure must be on file with Area International before any research may be released.

H2O-BOO2STŪ EQUINE OXYGEN uses E.P.A. certified purified distilled water. Purified water is produced by a suitable treatment process such as distillation, deionization, or reverse osmosis and meets the most recent definition of purified water in the United States Pharmacopeia. Distilled water is purified water that has been produced by vaporizing and then condensing water during the process of distillation. Distilled water is then stored in sterile 5 gallon PET bottles for later use.

H2O-BOO2STŪ EQUINE OXYGEN is manufactured from food grade certified sodium chloride, manufactured by Morton Salt, U.S.A., and distilled water in a proprietary modified electrolysis process.

H2O-BOO2STŪ EQUINE OXYGEN uses a surgical-grade stainless steel Digi-Fil Volumetric Digital Single Head Automated Filler for filling the above bottles.


NOTICE: This information is intended for educational purposes only and should not be construed as medical advice. These statements have not been evaluated by the Food and Drug Administration. These products are not intended to diagnose, treat, cure, or prevent any disease. Consult a qualified veterinarian before using any nutritional supplements on your horses.

This information may not be reproduced or utilized in any form or by any means, in whole or in part, electronic or mechanical, including photocopying, recording, or by any information storage and retrieval system, without prior written permission. 

Racing picture above is called “GALLOWAY & COMPANY”  from the Collection of Randy Galloway and Don Walker.